Title : Development of DNA markers to distinguish the Damascene rose from R. centifolia and R. gallica var. officinalis
Abstract:
The damask rose Rosa damascena Mill. is one of the most important ancient roses. It took its importance and fame and spread throughout the world due to its great aromatic, medicinal, cosmetic and nutritional benefits. Because of the great economic importance of R. damascena in Syria, and the fact that the morphology is not sufficient to confirm the identity of plant species, we used in this research a sample of the reliable damascene rose commercially bred in Bulgaria as a reference sample and PCR-based techniques to analyze the nuclear DNA (SSRs) and chloroplast DNA (DNA barcoding) in order to confirm the identity of the native R. damascena grow in Syria. The research also aimed to develop DNA-based markers that differentiate the Damascene rose from R. centifolia and R. gallica var. officinalis, which are close relatives to it. We were able to develop accurate DNA markers to distinguish the Damascene rose from R. centifolia and R. gallica var. officinalis. The results of this research can be used to ascertain the identity of any type of rose that is claimed to be R. damascena and to rationalize the Damascene rose revival program in Syria when expanding its cultivation and propagation in the country and when searching for the genetic resources of this rose in the country in order to protect them.
Audience Takeaways:
- They will learn how important to use DNA markers combined to morphology for accurate identification of rose species.
- The audience will improve the level of their job by using DNA markers for plant species identification.
- The research could be used to expand the audience research and teaching.
