Title : RNA-Seq analysis reveals important factors involved in the metabolic adaptation in liver of the transition cows
Abstract:
The transition period of a dairy cow, mostly defined as the period from week 3 antepartum until 3 week post-partum, is characterized by abrupt changes in physiology, metabolism, and nutrition of the animal. Intensive fetal growth before parturition, morphological and endocrine changes related to mammary gland development, and the tremendous increase in energy and nutrient demand with the onset of lactation are the most substantial challenges the dairy cow has to face during this period. Hence, optimal metabolic adaptation is required to avoid the development of metabolic and infectious diseases. The liver is the key organ controlling and regulating metabolic adaptation. Previous study revealed that liver glucose synthesis, cholesterol metabolism, lipogenesis, and inflammatory response are increased or activated during the transition in dairy cows, however, those works were limited by a low number of animals used or by the use of microarray technology, or both. To overcome such limitations, an RNA sequencing analysis was performed on liver biopsies from 20 Holstein cows. In our results we found 1,475 up regulated and 1,199 down regulated differently expressed genes with a false discovery rate adjusted P-value < 0.01 between pre-partum and post-partum dairy cows. Bioinformatics analysis showed an activation of the metabolism of lipid, glucose and amino acid with increased importance of the mitochondria and a key role of several signaling pathways, chiefly peroxisome proliferators-activated receptor and adipocytokines signaling. Fatty acid oxidation and gluconeogenesis, amino acid utilization to produce glucose, were among the most important functions revealed by the transcriptomic adaptation to lactation in the liver. The analysis also revealed high activation of cell proliferation but inhibition of xenobiotic metabolism, likely due to the liver response to inflammatory like conditions. Our data confirmed the importance of metabolic adaptation to lipid and glucose metabolism in the liver of early postpartum cows.
What will audience learn from your presentation?
- Metabolic adaption during transition period in dairy cows
- RNA-sequencing
- Dynamic impact approach
- Biomarkers of liver function during transition period
From the above listed information the participant of the conference will get experiences in data analysis using dynamic impact approach, on RNA sequencing and skill on liver transcriptomic adaption and etc.
