Title : Isolate of bacteria and fungi associated with dead honey bee (Apis mellifera) in certain Egyptian governorates and in vitro treating some of related bacteria by Nano propolis – chitosan
Abstract:
Apiaries of total 16 localities zone in Egypt (New valley, Minya, Gharbiya, Demyat, Sharqyia, Ismailia, Mansoura, Cairo, Suez, North Sinai, Banisweif, Qalubeiah, Giza, Behera, Alexandria and Alaqsour ) were investigated for diseases, whereas 30 adult workers honeybees were collected from the modern bee hives represent every locality and analyzed for microbial presence. It was found that dead bees in many cases occurred due to infection with the microorganisms; fungi were observed in four localities which were New valley, Mansoura, Cairo and North Sinai areas, while the bacteria were observed in the all localities except Alaqsur area, because the species of bacteria significantly increased fastly and spread inner and between honeybee colonies through the bee operations. By the microbial examination of bodies and guts of dead bees, it showed that presence of *Correspond author; Hashish M. E. {Email; [email protected]} Page 2 three fungal species (Rhizopus spp., Penicillium spp., and Saccharomyces cervisia), and presence of three species communities of bacteria in most of ecological localities, such as Bacillus spp., Escherichia coli and Pseudomonas spp. On the other hand, propolis loaded on Nano chitosan has laboratory proved its efficiency at very low concentrations against the isolated E. coli bacterium more than Bacillus spp.
