Induced mutagenesis is one of the most effective tools that has been employed considerably to produce inheritable variation as well as for identification of crucial nonsupervisory genes for economically important traits toward the crop enhancement. Mutations can be convinced by several ways similar as physical, chemical, and insertional mutagen treatments; still, these styles aren't preferred because of cost and tedious process. nevertheless, with the advancements in coming- generation sequencing( NGS) ways, millions of mutations can be detected in a veritably short period of time and, thus, considered as accessible and cost effective. likewise, convinced mutagenesis coupled with whole- genome sequencing has handed a robust platform for forward and rear inheritable operations. also, the vacuity of whole- genome sequence information for large number of crops has enabled target-specific genome editing ways as a preferable system to wangle asked mutations. The available genome editing approaches similar as ZFNs( Zinc Finger Nucleases), recap activator like effector nucleases( TALENS), and clustered regularly interspaced short palindromic reprises( CRISPR) CRISPR- associated9( Cas9) endonuclease have been employed to perform point-specific mutations in several factory species. In particular, the CRISPR/ Cas9 has converted the genome editing because of its simplicity and robustness, thus, have been employed to enhance biotic and abiotic stress resistance. The Special Issue of shops highlights the sweats by the scientific community exercising mutagenesis ways for the identification of new genes toward crop enhancement.