Title : Bioactive properties of some phenolic compounds fractionated from pomegranate peels crude juice
Abstract:
Phenolic compounds are secondary metabolites in plants and are considered as important natural molecules due to their bioactive properties. The crude juice of pomegranate peels the agro-food by-product was produced by the mechanical pressing without using a solvent. Moreover, phenolic compounds of the resultant crude juice were extracted by solid phase extraction (SPE) then fractionated by thin-layer chromatography (TLC) into their main component. These fractionated phenols were identified using several spectroscopic methods, i.e., Fourier-Transform Infrared Spectroscopy (FTIR), Mass spectrum (MS) and Nuclear Magnetic Resonance (NMR) spectroscopy. The fractionated polyphenols were subjected to the estimation of their antioxidant, antibacterial and anticancer potentials. Also, the cytotoxicity effects of these phenols on normal cells were estimated to check their safety. The results of FTIR revealed the presence of various functional groups such as OH, COOH, C=C, CHO and C=O. NMR and MS data showed that the five fractionated phenols were, gallic acid, ellagic acid, quercetin, catechin and vanillin. Ellagic acid exhibited strong potential to act as a metal chelator and higher reducing power activity while, vanillin showed high scavenging activity by DPPH method. Gallic and ellagic acids exhibited significant inhibitory activity against all bacterial strains under study when compared to other phenolic compounds. Catechin decreased the viability of MCF-7 breast adenocarcinoma cells and human colon cancer cells (HCT-116). Toxicological potential of phenolic compounds under study revealed no cytotoxic effects at the concentrations used in the present study. Accordingly, bioactive compounds, i.e., phenols from agro-food by-products which recovered using sustainable extraction way, can be used as raw material, or as novel products, with health benefits creating industries with added value.
Auidence Take Away Notes
- Agricultural and agro-food industrial processing produce a high amount of wastes and by-products which represent a vital issue for both environment and international economy since they are one of the causes for landfilling to be no longer sustainable. So, the main challenge nowadays is using these non-edible parts as a renewable and cheap source of added value bioactive compounds to introduce new, more sustainable, and natural alternatives to the market.
- Several extraction methods have been reported for the recovery of bioactive compounds that use organic solvents which may contribute to toxicity, presenting high volatility and non-renewability. Thus, there is a growing need for green and sustainable alternatives.
- Bioactive compounds, i.e., phenols from agro-food by-products which recovered using sustainable extraction way, can be used as raw material, or as novel products, with health benefits creating industries with added value.
